COMPARATIVE STUDY ON DISINFECTION EFFICACY OF GLUTARALDEHYDE AND PHTHALDEHYDE IN GASTROScopes
Since 2004, the Ministry of Health issued a standard for endoscopic cleaning and disinfection, glutaraldehyde disinfectant
It has been widely used in the cleaning and disinfection of gastroscope in medical institutions. However, due to the elimination of glutaraldehyde
The inherent toxicity of the poison itself and the improper use of the poison cause the length of the medical staff and patients.
Period or short-term health hazards occur from time to time..., And it is also gradually violent in endoscopic disinfection.
exposed many problems, in order to avoid the harm of glutaraldehyde to human health, to explore a more
Use, safe and efficient disinfection method, the hospital from October 2010 to November 2010
70 cases of gastroscopes after use were sterilized by glutaraldehyde immersion and ortho-phthalaldehyde immersion, respectively.
Soak 2 methods for disinfection, and compare the disinfection effect, is reported as follows.
1 Objects and Methods
1.1 research object
7O cases of Olympus gastroscope after gastroscopy (model
：
260,240), according to the random number method randomly divided into 2 groups, each group of 35 cases, respectively, using E.
dialdehyde soak for 10min, o-phthalaldehyde soak for 5r
ain for disinfection.
1.2 cleaning disinfectant
Legu Special Multi-enzyme Cleaning Solution for Special Endoscopes (Germany
General Laboratory);2% glutaraldehyde (Shanghai Medical Institute Pharmaceutical Co., Ltd.),Cidex o-benzene
Dimethylaldehyde High Level Disinfectant (OPA )[Johnson & Johnson (One L Sea) Medical Equipment Co., Ltd.
Division], containing positive phthalaldehyde 0.153, pH value is 7.45.
1.3 cleaning and disinfection methods manual cleaning and disinfection in strict accordance with the Ministry of Health "endoscopic cleaning and disinfection technology operationSpecification})2004 edition (hereinafter referred to as "specification") requires cleaning and disinfection, I .e. water washingEnzyme washing, cleaning, disinfection and repeated cleaning. When each step is changed, the stomach must be washed with a high-pressure air gun.Each lumen of the mirror is dried. Among them, during disinfection, the gastroscope after cleaning and drying was placed in 2%In the disinfection tank containing glutaraldehyde and OPA stock solution, each hole L channel is filled with disinfectant and soaked separately.bubble 10rain、5rain。
1.4 sampling method
The monitoring sampling site is the inner cavity surface of the endoscope, and 10 ml is taken for neutralization.
agent, injected from the entrance of the biopsy hole by sterile method, and recovered to the sampling tube at the exit, and
Submitted for inspection.
1.5 detection method
The test liquid shall be fully shaken with a vortex device, and then divided with a sterile straw.
Don't suck 0.5ml into 2 sterile plates with a diameter of 90mm, each plate
17ml of melted 47% nutrient agar was added respectively, and the agar was solidified and cultured at 35cC
After 48h, count; At the same time, inoculate on blood plate, Chinese blue plate and ss plate medium culture
To observe the growth of pathogenic bacteria. Disinfection qualified judgment standard is based on 《
disinfection technology
Specification (2002 edition): bacterial colony count <20cfu/piece, no pathogenic bacteria growth is
Qualified.
2 Results
Comparison of the disinfection effect of two kinds of disinfectants on gastroscope is shown in Table 1.
3 Discussions
3.1 digestive endoscopy as an invasive, minimally invasive diagnosis and treatment equipment, has been widely
for clinical use. Due to its special material, high precision and complex structure,
Washing and disinfection work is very difficult. Digestive endoscopy disinfection is not in place, will affect the patient's
Medical Security. As the only disinfectant in the Disinfection Technical Specification, glutaraldehyde has been
Widely used in endoscopic disinfection, due to the inherent toxicity of glutaraldehyde disinfectant itself.
and improper use of long-term or short-term health hazards to medical personnel and patients
It happened..., And the time of glutaraldehyde disinfectant is longer (10-45min),
Seriously affect the need for rapid reuse of endoscopes and reduce the disinfection of medical personnel
compliance, increase the safety risk of hospital cross infection. Therefore, we have 35 cases.
Endoscopic instruments after clinical examination were sterilized with 2% glutaraldehyde and treated
35 cases by phthalaldehyde disinfection of endoscopes for comparison, the results confirmed that both methods are
Safe and reliable, but the endoscope disinfection time is shortened from the original 10min to 5r.
Ain, big
The reuse rate of endoscopic instruments has been greatly improved, and the work efficiency of the endoscopy room has been improved.
Less waiting time for patients to check, improve patient satisfaction.
