Comparison of the effect of phthalaldehyde and glutaraldehyde on killing bacterial spores
Release time:
2022-09-27
Since o-phthalaldehyde (OPA) has been certified as a high-level disinfectant by the US Food and Drug Administration (FDA), it has been used for disinfection of endoscopes in foreign countries [1], and has been of great interest in China in recent years [～. In order to understand the ability of o-formaldehyde disinfectants to kill spores, we selected an imported o-formaldehyde disinfectant and a domestically produced strong glutaraldehyde disinfectant for comparison, and used a carrier-quantitative bactericidal assay to observe their efficacy in killing the spores of Bacillus subtilis black variant in the laboratory, and their stability in use. The results are reported below.
Phthalaldehyde (OPA) approved by the US Food and Drug Safety Administration(FDA) After certification of high-level disinfectant, it has been used for disinfection of endoscopes abroad [1], countryIn recent years, it has been very concerned about 【 ~. To understand the killing of o-phthalaldehyde disinfectantscapacity of spores, we chose imported o-phthalaldehyde disinfectant and domestic fortifiedComparison of glutaraldehyde disinfectant, using carrier quantitative sterilization test, its laboratory.The effect of killing Bacillus subtilis var. niger spores and the stability of use were observed.The results are reported below.
1 Materials and methods
1.1 material
Imported o-phthalaldehyde disinfectant (Johnson & Johnson), o-benzene two.The labeled content of formaldehyde is 0.50%~ 0.60%; the fortified glutaraldehyde disinfectant is hospitalProduct for use in disinfection of endoscopes with 2.0% of the glutaraldehyde label-2．2％,
1.2 physical and chemical indicators to detect the content of the active ingredients of disinfectants refer to the "disinfection technology."Specification> [4]Determination of products by high performance liquid chromatography for o-phthalaldehyde disinfectantsThe content of ortho-phthalaldehyde in the fortified glutaraldehyde disinfectant using sulfuric acid titrationDetermination of glutaraldehyde content in products; pH determination of disinfectant using PHS-25 precisionDigital pH meter.
1.3 carrier preparation
Spore suspension of Bacillus subtilis var. noir (ATCC 9372)liquid, using tryptone soy (TSB) nutrient broth to prepare a bacterial suspension for contamination. TestTest bacteria suspension 0.02rnl drop dye on sterile stainless steel disc (diameter 15mm, thickness
0．5rAm), put in a 37 ℃ incubator for drying, and make bacteria slices for later use.
1.4 neutralizer identification test
Press 《Sterilization Technical Specification> amp; Designs 6 GroupsBacillus subtilis black variant spores as test bacteria, using carrier quantitative sterilization test process.[5].
1.5 carrier immersion quantitative sterilization test will contain 5ml of disinfectant for each bacterial tablet.(positive control is phosphate buffer) sterile plate at (20±1)℃ constant temperatureIn the water bath, add the effect of bacterial tabletsI set the time, take out the bacteria slice and put it into 5mlIn the test tube of the mixture, the bacteria on the steel sheet are eluted by shaking for 40S with an electric mixer.and effect 10rain; Take the eluent for viable count and calculate the killing logarithm. AllThe test was repeated 3 times.
1.6 continuous service stability test
Take two 2000ml of phthalate respectivelyFormaldehyde and enhanced glutaraldehyde disinfectant stock solutions were contained in 2 sterile enamels with covers.Plate, each put clean medical forceps, scissors, hemostatic forceps and other medical equipment to reachFull load requirements, each Ft will be the instrument removed, washed with water, drained, and then soaked to disinfectionIn liquid. According to each carrier 10ml to soak the day, 7,14d enamel plate in the eliminationVenom to sterile plate, placed in (20±1)℃ constant temperature water bath 5rAin, will test bacteriaThe tablets were immersed in the carrier quantitative sterilization test.
2 knotsFruit
Determination of physical and chemical indexes of 2.1
o-phthalaldehyde disinfectant is a unilateral preparation, determinedContaining 0.53% ortho-phthalaldehyde, pH 7.45; enhanced glutaraldehyde disinfectantIs a compound preparation of glutaraldehyde and cationic surfactant, determined to contain glutaraldehyde
2.18, the pH value was 2.55 before activation, and the pH value was 7.74 after activation.
2.2 neutralizer identification test containing 3g/L lecithin, 3.0 Tween 8O,Phosphate buffer solution (PBS) of 1O L glycine as a neutralizing agent can effectivelyand phthalaldehyde disinfectant stock solution and enhanced glutaraldehyde disinfectant solution on the test bacteria.residual effect, and the neutralizer solution and its neutralization products on the black variant of Bacillus subtilis.There was no significant effect on the growth of spores.
Effect of 2.3 on killing bacterial spores Effect of ortho-phthalaldehyde disinfectant stock solution3h, the killing of Bacillus subtilis black variant spores on stainless steel sheet is3.21, the effect of 10h kill value> 6.10; enhanced glutaraldehyde disinfectant stock solutionAfter 0.5h of action, the killing of spores of Bacillus subtilis var.3.43, the killing logarithm value for 3h of action was> 6.10 (Table 1).
2.4 continuous service stability test
Continuous immersion of ortho-phthalaldehyde disinfectant stock solutionAfter soaking the medical device for 7 and 14 days, it acts for 3 hours to black Bacillus subtilis on the stainless steel sheet.The average killing of spores of the variant was> 3.00;After continuous soaking of medical devices for 7 and 14 days, the number of spores killed by the liquid for 3 hoursThe values are all> 6.o0 (Table 2).Table 1 Effects of two disinfectants on Bacillus subtilis var. niger at different timeComparison of spore killing effect
Note: The average number of bacteria in the positive control group was 1272000cfu/tablet. Results for 3 trialsAverage.
Table 2 Average killing logarithm values of two disinfectants with different action time
Note: The results are the average of 3 tests.
3 to askOn
The test results show that the effect of 0,53% o-phthalaldehyde disinfectant stock solution3h can achieve disinfection effect on spores, but with the enhanced effect of glutaraldehydeThere is a certain gap, which is basically consistent with Huang Yuhong's report [6]]. This o-phthalaldehyde eliminationThe results of killing spores by toxic agents are obviously not as good as those reported by Xu Nengfeng and others.【3] of domestic o-benzeneDimethyl aldehyde disinfectant, which may be imported as a unilateral preparation, while domestic o-phthalaldehyde elimination
The poison is mostly compounded with alcohols, quaternary ammonium salts and emulsifiers, and the pH value is above 8.0.On the related; Xu Nengfeng and others have confirmed that the surfactant-containing alkaline phthalic aldehyde kill.Spore effect is best.Because glutaraldehyde gradually exposed many problems in endoscopic disinfection, and before useTo activate, the use of relatively high concentration of defects, domestic and foreign scholars have proposed the use of benzene two.Formaldehyde disinfectant instead of glutaraldehyde for endoscopic disinfection [2,. This study proves that,o-phthalaldehyde disinfectant has a high level of disinfection effect, and the use of good stability, can be14d continuous use.In the experiment, it was found that the o-phthalaldehyde disinfectant would have ash after contacting the skin and clothes.The color reaction is not easy to wash away; The product description also says "Rinse correctly after disinfection."clean; may cause allergic reactions; contact with disinfectant may cause exposed skin orClothes dyeing; should avoid contact with eyes, skin or clothes ", should still causeAttention.
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