DETERMINATION OF UREA BY PHTHALDEHYDE METHOD
Release time:
2022-10-18
Urea is one of the first substances to be measured in body fluids.Master of LawThere should be diacetyl glyoxime method, urease method, enzyme coupling rate method, etc.The method is either boiling, or the reagent is more expensive at present, the market.
The supply of phthalaldehyde chromogenic method (OPA) determination of urea kit,The method does not need to boil, is simple, rapid, accurate, stable and easy to obtain. We react accordingprinciple, in the ISP-Urea determination on M semi-automatic biochemical analyzer,The effect is satisfied. Special report
1 Principle
In an acidic environment, urea in serum in the presence of_concubineThe action of o-phthalaldehyde generates a yellow substance, which is used in the production of metavanadate.Use it to turn into a blue substance. The depth of the color of the substance's liquid is within a certain range.
It is proportional to the serum urea concentration and is compared with the standard of the same treatment.The concentration of urea in the serum sample can be obtained by color determination.
2 Materials and methods
2.I reagent OPA assay urine cord kit} which includes liquid a BLiquid, urea standard solution, batch number 951102 of t of bo ding great wall clinical reagent co., ltd.Determination of urea by enzyme coupling rate method kit: Shanghai Changzheng Medical Science Co., Ltd.
Company, lot number 950828
2.2 instrument
IsP-M semi-automatic biochemical analysis only, model V/talobOne21。
2.3 Method Liquid A and Liquid B are mixed 10:1 before use. 25ul sampleAdd 1.1ml of mixed solution to this, 37"(3Fifteen minutes. In IsP-M semi-selfDetermination of end point method on dynamic biochemical analyzer, than blank mold, wavelength600m'The content of urea can be obtained by nt colorimetry. At the same time to blame amino acyl dehydrogenationAlcohol coupling reaction method, also in semi-automatic biochemical analysis only on dynamic creditsAnalysis.
3 Results and discussion
Author Unit, Inspection Department of Wuhe County Press Institute (233309)Reagents. But the price of imported reagents is more expensive, the use of domestic reagents or self-made reagents.Must achieve the same inspection effect as imported reagents, well-used sample quality control fluid.Calibration can be used beforeSeveral Problems of Cong Yulong in the Application of Hematology Analyzer ⅢIt is pointed out that the cell histogram not only provides diagnostic reference data for clinical, but also for the operation.The staff shall provide monitoring of the working status of the instrument and the reliability of the experimental results.control, it is generally necessary to determine whether microscopy is required after careful analysis of the histogram.The mirror examination report was sent again. We should continue to sum up experience and identify problems,Continuously improve the technical level of professionals to make it better for clinical treatment.Work Service
3.1 linear range in the range of 0~71.4mraol/I for the preparation of Lo speciesDifferent concentrations of urea solution, each concentration solution repeated determination of 3method in the urea dimension o ~ 28mraol/I has good linearity.
High, medium and low value urea (concentration of Lo．71, 5.36, 357mmol/I), measured 2O times, the batch CV is respectively31%, 23, 2.1 '6, with batch-to-batch CV of 4.2, 3.6,3．2％ 。
3.3 Recovery Experiment
at urea concentrations of 7.87 and 21.50mmol/In the serum sample of l, add urea at a concentration of 6.0 mmol/1., and then proceeddetermination, the recoveries were 90 and 98
Preparation of 513mmol/1 bile red cord and 7.6r in 3.4 interference experimentetool／1CHO, 3.5mmol/1 triglyceride, 4gA hemoglobin do interferenceCheck. It was found that there was no obvious interference (P>005)t-dimensional cord C concentration was lower
75mg/1 without interference (P>0.05) laboratory use of heavy ammonia reagent pairBenzenesulfonic acid can interfere with the reaction. Such substances should be prevented from being measured in the laboratory.Pollution.
OPA chromogenic method and each oxyacyl dehydrogenase couple for 3.5 accuracy experimentSimultaneous determination of serum specimens by combined rate method 50 inverted (hospital outpatient health examination), respectively, soil s is 5.18 1.70, 5.28±1.73. Statistics
treatment, there is no significant difference between the two methods (P. 0.05), and the correlation coefficient r is o.．9804. Regression equation Y a 1.0ix 0.0456.
In a word, the determination of urine by the o-phthalaldehyde method is convenient for the enzymatic method. CanAutomated analysis, inexpensive, stable reagents can be stored at room temperature, and the variation is small and dry.The linear range is good and highly correlated with enzymatic methods. Practice Certificate
This method is an economical and practical urea determination technique worthy of promotion.
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