Li Huihong, Zhang Chan

(Hangzhou Cancer Hospital, Hangzhou, China)310 2）

Abstract Objective To improve the cleaning method of multi-enzyme cleaning agent, improve the cleaning quality of oral cleaning equipment, the method will seriously pollute2,970 pieces of tooth extraction instruments were divided into 1 485 pieces in the test group and 1 485 pieces in the control group, and then divided into 3 groups, 495 pieces in each group, which were cleaned 0 h, 1 h and 2 h after pollution respectively. The test group was cleaned with 1:1 multi-enzyme cleaning agent heated to 25 ℃ and 40 ℃ for 5 mino with ultrasonic cleaning machine, and the control group was soaked with 1:1 multi-enzyme cleaning agent for 30 mino to remove the two groups respectively, rinse with running water. The residual occult blood detection method was used to detect the pilami hole, and the cleaning effect of each group was compared. Results The qualified rate of the test group and the control group which were cleaned immediately after pollution was 1%. The cleaning pass rate of the test group cleaned 1 h after pollution was 99-6%, and that of the control group was 99-6%.

97-0%, a significant difference between the two comparisons (P <0-01). The clean pass rate of the test group cleaned 2 h after pollution was 99-0%, and the clean pass rate of the control group was 99-0%.

1%, there was a significant difference between the two (P <0.01). Conclusion For the heavily contaminated tooth extraction instruments, the effect of ultrasonic cleaning by heating 25 ℃ and 40 ℃ with multi-enzyme cleaning agent is remarkable.

Key words multi-enzyme cleaning agent; ultrasonic cleaning; soaking cleaning; effect

CLC No:R187 Document Identification Code: B Article Number: 11-7658(2012)10-0937-02

There are many kinds of oral instruments, complex shapes, frequent use and serious pollution. And these devices directly contact the patient's blood, saliva, tissue fluid, etc., if the disinfection is not qualified, can become a variety of infectious diseases, easily cause hospital infection, and may even be infected with hepatitis B, AIDS and other infectious diseases. In order to explore how to quickly and effectively disinfect the seriously contaminated oral instruments, Hangzhou Cancer HospitalFrom January 2010 to January 20h, 2,970 contaminated oral extraction instruments were taken and cleaned after 0 h, 1 h and 2 h respectively. Multi enzyme cleaning agent is divided into ultrasonic cleaning and immersion cleaning. Compare the cleaning effect of different time and method.

1 Materials and methods

1.1 Materials

Tooth extraction equipment used by patients (extraction forceps, curette, bone chisel, needle holder, tooth stents, etc.), ultrasonic cleaning machine (Ningbo Feister Equipment Company, model 12006), Conway Dado Enzyme Endoscope Cleaning Agent

1. 2 Method

will be seriously polluted2,970 pieces of tooth extraction instruments were divided into 1 485 pieces in the test group and 1 485 pieces in the control group, and then divided into 3 groups, 495 pieces in each group, which were cleaned at 0 h, 1 h and 2 h after pollution respectively. The test group used 1:100 multi-enzyme cleaning agent to heat the contaminated tooth extraction instruments to 25 ℃ -40 ℃ for 5 mino. The control group used 1:1 multi-enzyme cleaning agent to soak for 30 mino to take out the two groups of tooth extraction instruments respectively, washed them with flowing water, and detected by the occult blood detection method of Pilami cave. Wipe the test strip on the test card against the oral appliance, then turn the back of the test card up and open the cover of the "Development Window. Drop 1 drop of Developer A (Zhuhai Besso Biotechnology Co., Ltd.) on the left and right sides of the square. After the reagent has completely penetrated, drop 1 drop of Developer B on each side. When human B reagent is added, light purple red will gradually be produced within 2 min, and the report is positive. Negative reactions were reported without any purple-blue or purple-red color reactions.

2 Results

The qualified rate of cleaning of the test group and the control group of the tooth extraction instrument immediately after contamination was1%. The cleaning pass rate of the test group cleaned 1 h after contamination was 99-6%, and that of the control group was 97-0%, with a significant difference between the two (P <0-01). The qualified rate of cleaning in the test group was 99-0% and that of the control group was 9L1% (P <0.01).

3 Discussions

Residual blood affects the disinfection and sterilization effect of tooth extraction instruments. Any residual organic substances, such as blood clots, pus, protein mucous membrane will hinder the effective contact between microorganisms and sterilization media, and will produce a protective film of bacteria and affect the sterilization effect, resulting in sterilization failure. Therefore, the correct cleaning process is very important. Tooth extraction instruments are difficult to clean due to their complex shape and structure. In particular, the complex dry blood formed by the untimely treatment of contaminated instruments is a difficult problem in cleaning and disinfection. For test group1:100 multi-enzyme cleaning agent is heated to 25 ℃ and 40 ℃, and the cleaning effect is remarkable after immediate cleaning, 1 h cleaning and 2 h cleaning, which reduces the existence of residual blood of instruments and improves the cleaning quality of tooth extraction instruments. The control group was soaked with 1:100 multi-enzyme detergent for 30 min, and the qualified rate of immediate cleaning was 1%, but the cleaning effect after 1 h and 2 h was significantly worse than that of the experimental group. Experiments show that the oral tooth extraction equipment should be cleaned immediately after use.

The multi-enzyme cleaner enhances the cleaning effect in the effective temperature. Enzymes are large protein molecule catalysts that can be used to accelerate chemical processes. multi-enzyme in25 ℃ 0 ℃ temperature pH value close to the neutral point under the condition of the best effect. If the temperature is too low, the soaking time should be extended. Excessively high temperatures can result in loss of enzyme activity, reduced enzyme efficacy, and coagulation of proteins on the surface of the device. Ultrasonic cleaning can effectively control the temperature, and accompanied by mild shock, more effectively accelerate the decomposition of dirt into debris, from the surface of the article. Its anti-deposition effect makes the immersed instrument easy to rinse. Soaking cleaning due to low temperature, to extend the soaking time, the solution contains a variety of proteolytic enzymes, cleaning all kinds of organic dirt, are dissolved in the cleaning fluid. On the one hand, the enzyme in the cleaning liquid is consumed, on the other hand, the cleaning liquid itself has no disinfection function. Various pathogenic microorganisms gather in the cleaning solution for a long time to cause new pollution. The surface of the tooth extraction instrument cleaned by the experimental group is clean and bright, and pollution-free, which is an ideal cleaning method, which can effectively ensure the cleaning quality of the oral tooth extraction instrument and control the hospital infection.