To Explore the Effect of Phthalaldehyde on Digestive Endoscope Simulation Field Disinfection

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Objective To study and analyze the anti-virus sterilization effect of phthalaldehyde (OPA) on digestive endoscopy simulation site. Methods The suspension sterilization test method and the simulated field disinfection test were used. Methods, the anti-virus effect of phthalaldehyde on digestive endoscopy was observed and analyzed. Results The content of ortho-phthalaldehyde in ortho-phthalaldehyde disinfectant was 5500 mg/L. The neighborhood. The concentration of the original solution of the benzene aldehyde disinfection solution was 2500 mg/L to kill the Escherichia coli, Pseudomonas aeruginosa and Staphylococcus in the suspension for three minutes, and the killing value was more than 5.0. Neighbor Candida albicans and Mycobacterium chelonae subspecies abscess in suspension were killed for three minutes at a concentration of 2800 mg/L in the benzene-formaldehyde disinfectant, with a killing logarithm of> 4.0. Right. The number of inactivation pairs of poliovirus in the suspension was> 4.0. The ortho-phthalaldehyde stock solution concentration of 5500 mg/L disinfectant, in the digestive endoscope for 20 minutes, the digestive The killing of Pseudomonas aeruginosa in the lumen of the mirror simulation was> 5.0. Conclusion o-phthalaldehyde disinfectant can kill bacteria and viruses in suspension quickly and effectively, digestive endoscopy simulation The effect of field disinfection is good.

0 Introduction O-phthalaldehyde is a kind of pharmaceutical intermediate. In recent years, there are many studies on this new type of disinfectant at home and abroad. It is generally used to sterilize the endoscopes of surgical instruments or equipment in hospitals. In the domestic market, o-phthalaldehyde disinfectant products are rarely observed for their anti-virus and sterilization effects after review [1]. In this study, a disinfectant solution containing 5500 mg/L phthalaldehyde was used, and its pH value was 7.39. Observe the effect of o-phthalaldehyde disinfectant on killing microorganisms and viruses and the effect of simulated field disinfection. The objects of this study include bacterial propagules, fungi, mycobacteria and viruses, and observe the disinfection of digestive endoscopy simulation field.

1 Data and methods

The ortho-phthalaldehyde disinfectant used in the 1.1 of experimental data has a stock solution content of 5500 mg/L and a pH value of 7.39. The type of endoscope automatic cleaning and disinfection machine used in the experimental test is ASPENDOCLENS-NSX-TM, which is produced by Johnson & Johnson Company of the United States. The company is currently the largest medical and health care product company in the world. The test subjects included Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans, Mycobacterium chelonae subspecies abscess, and poliovirus. The carrier of infectious bacteria used in the digestive endoscopy simulation site is polytetrafluoroethylene with a diameter of 6mm, which is divided into small segments and used for disinfection, with a total length of 1800mm.

1.2 experimental method (1) the production of bacterial suspension, take each different strains isolated and cultured colonies, nutrient agar inoculation, Candida albicans inoculation, cultured in an environment of 37 degrees Celsius for 24 hours, and then washed with physiological saline under the sloping surface of the bacteria moss, diluted to make bacterial suspension. Refer to the relevant requirements of the "Technical Specification for Inspection of Disinfection Effect of Endoscope Cleaning and Disinfecting Machines" issued by the Ministry of Health for cleaning and testing, and the specific steps of cleaning and disinfecting machines. The polio virus suspension was cultured into a monolayer with MEM cells of calf serum, and the titer was determined after the virus was obtained, and the virus suspension was prepared. (2) Quantitative sterilization of the suspension was carried out at an ambient temperature of 20 degrees Celsius. Add 1 ml of bacterial suspension and 5 ml of phthalaldehyde disinfectant solution into the test tube and mix them for action. The test was repeated for 3 times, and the killing value was finally calculated. (3) The experiment of killing virus should be carried out at 20 degrees Celsius. 0.3 ml of virus suspension and 0.7 ml of phthalaldehyde are added into the test tube to mix and react. Neutralizer is used to mix evenly. After neutralization for 10 minutes, the pathological changes are observed. The experiment is repeated for 3 times. Finally, the killing logarithm is calculated. (4) Digestive endoscopy simulation on-site disinfection, first the bacterial suspension pollution, select Pseudomonas aeruginosa as a reference index. The digestive endoscopy was cleaned and disinfected, and the anti-virus effect of o-phthalaldehyde was sampled. Pour phthalaldehyde disinfectant into the endoscope, place the digestive endoscope on the disinfection machine, run a disinfection cycle, and detect the surviving natural bacteria after the end. After collecting the eluent, send it for inspection, determine and calculate the killing logarithm value.

2 Results The results showed that the ortho-phthalaldehyde disinfectant solution with a concentration of 2500 mg/L had a significant killing effect on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, and the killing pairs of Candida albicans and Mycobacterium abscess subspecies were all> 4.

3 Discussion o-phthalaldehyde is mainly through the reaction with the microbial protein structure of active molecules to produce a black phenomenon, thus forming the mechanism of action on microorganisms [2]. The experimental results show that the sterilization effect of o-phthalaldehyde is very strong, and its ratio of low concentration requirements, low corrosion and irritation and other advantages are very significant. It can quickly kill bacteria, Mycobacterium chelonae, viruses and other microorganisms, and the disinfection effect is thorough and reliable. In the field simulation test of endoscopic cleaning, the cleaning runs for a cycle, and the residual viruses and bacteria are within the qualified range. Endoscope cleaning and disinfection machine with phthalaldehyde disinfection solution, after cleaning, the disinfection effect of endoscopes through the test are in the range of standards, phthalaldehyde has a certain coloring irritation, with the use of cleaning machine is more ideal. Will be cleaned in the new type of selective anticholinergic drugs, for a variety of receptors can produce prominent antagonistic effect, by directly in the central, peripheral nervous system to play an anticholinergic effect. The combination of multiple regimens can not only relieve the patient's condition in a shorter time, but also ensure the safety. Conclusion The combination therapy for patients with acute organophosphorus poisoning can not only make the clinical indicators enter the normal range in a shorter time, but also has high safety, which is of great significance to the prognosis of patients, and is worthy of clinical promotion.

References [1]? Lao Xinyuan. Study on the Effect of Comprehensive Treatment and Nursing for Patients with Severe Organophosphorus Pesticide Poisoning Complicated with Respiratory Failure [J]. Contemporary Medical Review, 2015,13(24):81-82. [2]? Lin Tianlai, Huang Minglian, Wei Sican. Clinical Study on Atropine and Penehyclidine Combined with Hemoperfusion in the Treatment of Severe Organophosphorus Insecticide Poisoning [J]. China Modern Drug Application, 2016,10(12):156-158.


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