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Hepatitis B is one of the main common clinical infectious diseases, which is harmful to the human body. If the gastroscope of patients with hepatitis B cannot be thoroughly disinfected, it will cause serious consequences.... Therefore, the selection of good effect and short time required endoscopic disinfectant is the basic standard for gastroscope disinfection. To this end, the hospital will be o-phthalaldehyde on the pollution of hepatitis B virus gastroscope disinfection effect and the impact on HBV-DNA content were studied and analyzed, the specific report is as follows. 1 Data and Methods 1.1 General Data 168 sets of gastroscopes of 168 patients with viral hepatitis B from January 2016 to October 2017 in our hospital were randomly selected by computer and divided into study group and control group, 84 sets each. There were 43 males and 41 females in the study group, aged 38-65 years old, average age f 51.5±5.2) years old. In the control group, there were 40 males and 44 females, aged 38-66 years old, with an average age of 52.0±5.3 years old. The difference between the two groups of general data is not statistically significant fP. 0.o5), comparable. 1.2 Methods Two groups of patients with hepatitis B virus infection after gastroscopy, gastroscope cleaning and disinfection. The research group disinfected the gastroscope with phthalaldehyde disinfectant [Shandong Dr. Xiao Disinfection Technology Co., Ltd., approval number: Lu Wei Xiao Zheng Zi (2011) No. 0029), specification: 2.5L/barrel, phthalaldehyde content: 0.5-0.6]. In strict accordance with the requirements of the "Technical Operation Specification for Endoscopic Cleaning and Disinfection" issued by the State Ministry of Health (the current Commission for Planning and Health), the operation is carried out for 20min, and the special RUHOF full-effect multi-enzyme for endoscopes is used for cleaning. The control group was soaked and disinfected with 2% enhanced glutaraldehyde [Shandong Lierkang Disinfection Technology Co., Ltd., approval number: Wei Xiao Zi f2000) No. 0059), specification: 500ml/bottle, glutaraldehyde content: 2.0~2.2]; Disinfection standards, methods and cleaning are the same as those of the study group. Then two groups of specimens were collected for detection, and the time was 3min and 5min. 1.3 the observation index, the average content of PCR fluorescence quantitative and qualitative detection HBV-DNA in the cavity of gastroscope after cleaning and disinfection was observed and compared between the two groups. 1.4 statistical methods SPSS22.0 statistical software was used for data statistical analysis. The measurement data are expressed as mean s. standard deviation (s), using t-test, and the counting data are expressed as rate (%), using the test. PO. 05); the average content of PCR fluorescence quantitative and qualitative detection of HBV-DNA (5.89±0.28)X10, (5.32±1.28) * 10 in the study group of 5min disinfection. significantly lower than the control group (8.34±0.65)X10, (8.34±1.36)× 10, the difference was statistically significant (P<0.o5).

3 Discussion According to research and investigation, the incidence of iatrogenic infection caused by unqualified gastroscope cleaning and disinfection in China has continued to rise in recent years, posing a serious threat to the physical and mental health of patients J. Especially for gastroscopes contaminated with hepatitis B virus, if disinfection is not complete, it will cause serious cross-infection and eventually lead to hepatitis infectious virus E4]o Therefore, strengthening the selection criteria and prevention and control of endoscopic disinfectant is the key J to reduce the cross-infection rate of gastroscopes. In recent years, the clinical use of phthalaldehyde for disinfection of contaminated hepatitis B virus gastroscope, through the test found that its disinfection effect is good. In this study, our hospital will be o-phthalaldehyde on the pollution of hepatitis B virus gastroscope disinfection effect and the effect of HBV-DNA content were studied and analyzed, and with the clinical commonly used to strengthen the effect of glutaraldehyde were compared. The results showed that there was no significant difference in the contents of PCR fluorescence quantitative and qualitative detection HBV-DNA in the gastroscope cavity between the two groups before and after cleaning and 3min after disinfection (P>O.05). The average contents (5.89±0.28)× 10, (5.32±1.28)× 10 of PCR fluorescence quantitative and qualitative detection of HBV-DNA in the gastroscope cavity of the study group were significantly lower than those (8.34±0.65)× 10, (8.34±1.36)× 10 in the control group, the difference was statistically significant (corpse <0.05). It can be seen that the disinfection effect of o-phthalaldehyde is remarkable, and the reason why o-phthalaldehyde can achieve good results is mainly because the disinfectant has the advantages of low concentration, high efficiency and rapid disinfection, and can achieve high level disinfection in 5min. It is also simple and convenient to use without activation, mixing or dilution. And it is a non-toxic safety product, which better protects the health of the cleaning and disinfection medical staff, and at the same time has no corrosive damage to the equipment.... Conclusion The effect of phthalaldehyde on the disinfection of contaminated hepatitis B virus gastroscope is significant, which can effectively reduce the content of HBV-DNA in the endoscopy cavity and improve the efficiency of the use of endoscopy, which is worthy of clinical application. References Fu Chengfeng, Zheng Dequan, Zeng Xiong. Comparison of the efficacy of glutaraldehyde and phthalaldehyde in the disinfection of gastroscopy. Modern Digestive and Interventional Diagnosis and Treatment, 2016,12(4):48-50. Liang Xueyue, Zhang Xiaoyan, Tang Lianhao. Comparison of Disinfection Effect and Economic Benefit of Phthalaldehyde and Glutaraldehyde on Digestive Endoscope. Massage and Rehabilitation Medicine, 2014,3(7):138. Shi Yuai, Zheng Meiying, Zhang Meiling. Comparison of the efficacy of glutaraldehyde and phthalaldehyde in the disinfection of gastroscopes. Contemporary Nurses (Academic Edition),201l,10(12):29-30. Qin Juanwen, Lu Jiefu, Zheng Chaowei, et al. Comparison of efficacy of ortho-phthalaldehyde and glutaraldehyde in disinfection of contaminated hepatitis B virus gastroscope. Nursing Research, 2017,31(1): 97-99. Ma Chunli. Experimental study on the disinfection effect of three kinds of disinfectants on bacteria and hepatitis B virus in digestive endoscopy. The Fourth Military Medical University, 2013. Song Xiangyang, Li Wuping, Ma Chunli, et al. Experimental study on the effect of three kinds of disinfectants on the removal of hepatitis B virus from gastroscope. Chinese Journal of Disinfection, 2015,32(3):232- 234. Li Jinlan. Discussion on the use of o-phthalaldehyde and glutaraldehyde in Xiao venom. China Health Nutrition, 2016,26(27):303-304. [Date of receipt: 2017-12-12]